First detection of a plasmid-encoded New-Delhi metallo-beta-lactamase-1 (NDM-1) producing Acinetobacter baumannii using whole genome sequencing, isolated in a clinical setting in Benin

Abstract Background Carbapenem-resistant Acinetobacter baumannii is considered a top priority pathogen by the World Health Organization for combatting increasing antibiotic resistance and development of new drugs. Since it was originally reported in Klebsiella pneumoniae 2009, quick spread bla NDM-1 gene encoding New-Delhi metallo-beta-lactamase-1 (NDM-1) increasingly recognized as serious threat. This usually carried large plasmids has already been documented diverse bacterial species, including A. . Here, we report first detection NDM-1-producing strain isolated Benin. Case presentation A 31-year-old woman admitted to surgical unit with diagnosis post-cesarean hematoma. An extensively-drug resistant solely susceptible amikacin, colistin ciprofloxacin, several other antibiotics ceftazidime, imipenem, meropenem, gentamicin, tobramycin, ceftazidime/avibactam, sulfamethoxazole-trimethoprim, from wound. Production demonstrated immunochromatographic testing. Whole genome sequencing isolate confirmed presence , but also genes against multiple beta-lactamases classes antibiotics, addition virulence genes. Moreover, found be present Tn125 transposon integrated on plasmid. Conclusions The discovery this carrying Benin worrying, especially because its high potential risk horizontal transfer due being into located Strict control prevention measures should taken, once positive identified prevent Enterobacterales. Capacity building required governmental agencies provide suitable treatment options strategies, combination strengthening laboratory services surveillance pathogen.